brain heart infusion bhi broth Search Results


brain heart infusion bhi broth  (MedChemExpress)
94
MedChemExpress brain heart infusion bhi broth
Figure 5. Lactate and <t>A.</t> <t>muciniphila</t> regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of <t>BHI</t> medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.
Brain Heart Infusion Bhi Broth, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/brain+heart+infusion+bhi+broth/Brain+Heart+Infusion+Broth/pm39782002-58-10-20
Average 94 stars, based on 1 article reviews
brain heart infusion bhi broth - by Bioz Stars, 2026-09
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brain heart infusion bhi broth  (Bio-Rad)
95
Bio-Rad brain heart infusion bhi broth
Figure 5. Lactate and <t>A.</t> <t>muciniphila</t> regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of <t>BHI</t> medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.
Brain Heart Infusion Bhi Broth, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
brain heart infusion bhi broth - by Bioz Stars, 2026-09
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brain heart infusion media  (Valiant Co Ltd)
94
Valiant Co Ltd brain heart infusion media
Figure 5. Lactate and <t>A.</t> <t>muciniphila</t> regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of <t>BHI</t> medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.
Brain Heart Infusion Media, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
brain heart infusion media - by Bioz Stars, 2026-09
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brain heart broth bh  (Chemie GmbH)
90
Chemie GmbH brain heart broth bh
Figure 5. Lactate and <t>A.</t> <t>muciniphila</t> regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of <t>BHI</t> medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.
Brain Heart Broth Bh, supplied by Chemie GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
brain heart broth bh - by Bioz Stars, 2026-09
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brain heart infusion (bhi) broth  (Carl Roth GmbH)
90
Carl Roth GmbH brain heart infusion (bhi) broth
Figure 5. Lactate and <t>A.</t> <t>muciniphila</t> regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of <t>BHI</t> medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.
Brain Heart Infusion (Bhi) Broth, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/brain+heart+infusion+bhi+broth/brain+heart+infusion++bhi++broth/pm36671363-198-10-15
Average 90 stars, based on 1 article reviews
brain heart infusion (bhi) broth - by Bioz Stars, 2026-09
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heart infusion broth bhi broth  (HiMedia Laboratories)
90
HiMedia Laboratories heart infusion broth bhi broth
Figure 5. Lactate and <t>A.</t> <t>muciniphila</t> regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of <t>BHI</t> medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.
Heart Infusion Broth Bhi Broth, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
heart infusion broth bhi broth - by Bioz Stars, 2026-09
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brain heart infusion broth bhi broth  (Eiken Chemical)
90
Eiken Chemical brain heart infusion broth bhi broth
Figure 5. Lactate and <t>A.</t> <t>muciniphila</t> regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of <t>BHI</t> medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.
Brain Heart Infusion Broth Bhi Broth, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/brain+heart+infusion+bhi+broth/brain+heart+infusion++bhi++broth/us09394572-429-77-83
Average 90 stars, based on 1 article reviews
brain heart infusion broth bhi broth - by Bioz Stars, 2026-09
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brain heart infusion (bhi) broth  (RPI Inc)
90
RPI Inc brain heart infusion (bhi) broth
Figure 5. Lactate and <t>A.</t> <t>muciniphila</t> regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of <t>BHI</t> medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.
Brain Heart Infusion (Bhi) Broth, supplied by RPI Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/brain+heart+infusion+bhi+broth/brain+heart+infusion++bhi++broth/pm39393642-46-15-21
Average 90 stars, based on 1 article reviews
brain heart infusion (bhi) broth - by Bioz Stars, 2026-09
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brain heart infusion (bhi) agar  (PanReac AppliChem)
90
PanReac AppliChem brain heart infusion (bhi) agar
Figure 5. Lactate and <t>A.</t> <t>muciniphila</t> regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of <t>BHI</t> medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.
Brain Heart Infusion (Bhi) Agar, supplied by PanReac AppliChem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brain heart infusion broth (bhib  (Lab M Ltd)
90
Lab M Ltd brain heart infusion broth (bhib
Figure 5. Lactate and <t>A.</t> <t>muciniphila</t> regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of <t>BHI</t> medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.
Brain Heart Infusion Broth (Bhib, supplied by Lab M Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brain heart infusion (bhi) mediumcontaining plates  (bioTRADING Benelux B.V)
90
bioTRADING Benelux B.V brain heart infusion (bhi) mediumcontaining plates
Figure 5. Lactate and <t>A.</t> <t>muciniphila</t> regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of <t>BHI</t> medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.
Brain Heart Infusion (Bhi) Mediumcontaining Plates, supplied by bioTRADING Benelux B.V, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/brain+heart+infusion+bhi+broth/brain+heart+infusion+broth++bhi/pm37888617-303-7-13
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brain heart infusion (bhi) mediumcontaining plates - by Bioz Stars, 2026-09
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brain heart infusion broth pras-bhi  (Becton Dickinson)
90
Becton Dickinson brain heart infusion broth pras-bhi
Figure 5. Lactate and <t>A.</t> <t>muciniphila</t> regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of <t>BHI</t> medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.
Brain Heart Infusion Broth Pras Bhi, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 5. Lactate and A. muciniphila regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of BHI medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.

Journal: Gut microbes

Article Title: Gut-derived lactic acid enhances tryptophan to 5-hydroxytryptamine in regulation of anxiety via Akkermansia muciniphila .

doi: 10.1080/19490976.2024.2447834

Figure Lengend Snippet: Figure 5. Lactate and A. muciniphila regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of BHI medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.

Article Snippet: Akkermansia muciniphila (A. muciniphila, ATCC BAA-835) was anaerobically cultured in brain heart infusion (BHI) broth supplemented with 0.5% gastric mucin (MCE, New Jersey, USA) at 37°C.

Techniques: Western Blot, Expressing, Quantitative RT-PCR, Concentration Assay, Activity Assay